Відео

This is research paper of anyone or ...u just made a video to give information plz tell?

How to accumulate microplastic into zebrafish in lab for toxicological study? That is either by adding plastic directly to the water or adding plastic powder to the feed or other way which is more authentic?

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Subscribe, yes I’m talking to you, you plk83 student

Great Presentation!

Super well made video! Thank you!

Beautiful information

How do we plot our values to determine LC50 results? Linear regression? Sigmoidal curve?

Very informative! - Could you expand on how to calculate the LD50 value? -What do you think of the Celltox green assay?

I want to talk to a professional scientist like yourself and see if you are aware of the experiments being done in the 1920's to feed pineal glands to baby animals which made them grow extremely fast in all species? Also are you aware that some occult secret societies eat human pineal glands in rituals? What are the connections?

Clear and well-explained! I wonder if these techniques will work in my experiment using a chemical to inhibit LDHA in tumors, especially alamar blue!

This lecture was great. Thank you sir

Thank you.

New subscriber. Very happy to be here!

thanks for your lecture

Thanks so much Sir!

Hello Sir, So if i want to use "comparative ct" for my research, i shouldn't use standard curve, is it true? Thanks in advance.

clear to understand. thx

Thank u so much Sir... :)

Super relevant and easy to understand! Thank you so much sir! 🥰💕 More power!

Thank you so much. Wonderful

Indeed this is useful

Hello doctor, thanks for this lecture I am doing rt PCR for COVID19. First extraction and then amplification using primers for R gene, N gene and RdRb gene. Can you help me with the method validation it is qualitative method. Thank you

I emailed you, hope you will see my message and answer !

That's great.

Mr King, I would like to make you some questions. I am going to start a study to determine the cytotoxicity in vivo of iron alloyed with other metals. could you , if possible, to share your mail ? or, otherwise, to contact me, please.. mademasalud@hotmail.com. wsp: +573214955578. I really appreciate your time, attention and help. .... and thanks for the lectures.

i have a question please answer me can i perform cytotoxic assay on cell directly especially leukemic cell without culturing it

Another question is , i noticed that when they do the Alamer Blue test: they add cell and copper sulfate first (4 h), then add Alamer Blue for 2h (for 6 hours group) and 20h (for the 24 hours group). But for the MTT test: they add cell and copper sulfate first (4 h) then add MTT for 2h for 6 hours group. but for the 24 hours group, they left cell and copper sulfate for 22h and then add MTT just for 2 hours. why they dont use the same time method as Alamer Blue test? is that the same reason that ALamer blue is not sensitive to be influenced by the copper sulfate, so we can add it earlier? thank you!

Thank you for your lecture! could you help to explain the MTT VS Alamer blue part. you said Alamer shows better result because copper sulphate has strong reductive power, so MTT approach may not be able to counteract the effects of the copper ions. i am so confused about this, so basically, live cells can produce a reductive items which can reduce MTT to from a purple product. copper sulphate has strong reductive power as well, so it should reduce MTT to from a purple product, that is why the cells all died out shown on the graph. (but actually there should still have cells alive just because MTT reduced by copper sulphate rather than the real reductive chemicals metabolised by live cells right?) so i am wondering, Alamer blue show better result, does it mean the Alamer blue is not sensitive to reduced by copper sulphate, so Alamer blue still get reduced by real reductive chemicals metabolised by live cells, namely NADPH or NADH. if it is true then why? and also for the reducing agents for MTT, are they NADPH or NADH as well? Thank you very much if you can help me !

Sir, do you give some more inforormation or video about cadmiym effect on rice plant?

Sir really great

I recommend looking at the cell viability assay chapter in the NIH “Assay Guidance Manual”. It goes into more assays and more details than this set of videos. Just Google Assay Guidance Manual NIH. It is open access and available for teaching. It corrects some of the errors in these videos.

Thank you for the explanations!

Great, thanks alot

Dear sir,where can i find this lecture as a PPTs ?

Hi, could you give examples of assays of membrane testing? Thank you in advance